Heavy metal accumulation by Saccharomyces cerevisiae cells armed with metal binding hexapeptides targeted to the inner face of the plasma membrane.
Identifieur interne : 000090 ( Main/Exploration ); précédent : 000089; suivant : 000091Heavy metal accumulation by Saccharomyces cerevisiae cells armed with metal binding hexapeptides targeted to the inner face of the plasma membrane.
Auteurs : Lavinia Liliana Ruta [Roumanie] ; Ralph Kissen [Norvège] ; Ioana Nicolau [Roumanie] ; Aurora Daniela Neagoe [Roumanie] ; Andrei José Petrescu [Roumanie] ; Atle M. Bones [Norvège] ; Ileana Cornelia Farcasanu [Roumanie]Source :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2017.
Descripteurs français
- KwdFr :
- Dépollution biologique de l'environnement (MeSH), Membrane cellulaire (composition chimique), Membrane cellulaire (métabolisme), Métaux lourds (métabolisme), Oligopeptides (composition chimique), Oligopeptides (génétique), Oligopeptides (métabolisme), Phénotype (MeSH), Protéines à fluorescence verte (composition chimique), Protéines à fluorescence verte (génétique), Régulation de l'expression des gènes (MeSH), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (métabolisme).
- MESH :
- composition chimique : Membrane cellulaire, Oligopeptides, Protéines à fluorescence verte.
- génétique : Oligopeptides, Protéines à fluorescence verte, Saccharomyces cerevisiae.
- métabolisme : Membrane cellulaire, Métaux lourds, Oligopeptides, Saccharomyces cerevisiae.
- Dépollution biologique de l'environnement, Phénotype, Régulation de l'expression des gènes.
English descriptors
- KwdEn :
- Biodegradation, Environmental (MeSH), Cell Membrane (chemistry), Cell Membrane (metabolism), Gene Expression Regulation (MeSH), Green Fluorescent Proteins (chemistry), Green Fluorescent Proteins (genetics), Metals, Heavy (metabolism), Oligopeptides (chemistry), Oligopeptides (genetics), Oligopeptides (metabolism), Phenotype (MeSH), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (metabolism).
- MESH :
- chemical , chemistry : Green Fluorescent Proteins, Oligopeptides.
- chemistry : Cell Membrane.
- chemical , genetics : Green Fluorescent Proteins, Oligopeptides, Saccharomyces cerevisiae.
- metabolism : Cell Membrane, Metals, Heavy, Oligopeptides, Saccharomyces cerevisiae.
- Biodegradation, Environmental, Gene Expression Regulation, Phenotype.
Abstract
Accumulation of heavy metals without developing toxicity symptoms is a phenotype restricted to a small group of plants called hyperaccumulators, whose metal-related characteristics suggested the high potential in biotechnologies such as bioremediation and bioextraction. In an attempt to extrapolate the heavy metal hyperaccumulating phenotype to yeast, we obtained Saccharomyces cerevisiae cells armed with non-natural metal-binding hexapeptides targeted to the inner face of the plasma membrane, expected to sequester the metal ions once they penetrated the cell. We describe the construction of S. cerevisiae strains overexpressing metal-binding hexapeptides (MeBHxP) fused to the carboxy-terminus of a myristoylated green fluorescent protein (myrGFP). Three non-toxic myrGFP-MeBHxP (myrGFP-H6, myrGFP-C6, and myrGFP-(DE)3) were investigated against an array of heavy metals in terms of their effect on S. cerevisiae growth, heavy metal (hyper) accumulation, and capacity to remove heavy metal from contaminated environments.
DOI: 10.1007/s00253-017-8335-0
PubMed: 28577027
Affiliations:
Links toward previous steps (curation, corpus...)
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Panduri 90-92, Bucharest, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>Faculty of Chemistry, University of Bucharest, Sos. 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Panduri 90-92, Bucharest, Romania. ileana.farcasanu@chimie.unibuc.ro.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>Faculty of Chemistry, University of Bucharest, Sos. 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In an attempt to extrapolate the heavy metal hyperaccumulating phenotype to yeast, we obtained Saccharomyces cerevisiae cells armed with non-natural metal-binding hexapeptides targeted to the inner face of the plasma membrane, expected to sequester the metal ions once they penetrated the cell. We describe the construction of S. cerevisiae strains overexpressing metal-binding hexapeptides (MeBHxP) fused to the carboxy-terminus of a myristoylated green fluorescent protein (myrGFP). Three non-toxic myrGFP-MeBHxP (myrGFP-H6, myrGFP-C6, and myrGFP-(DE)3) were investigated against an array of heavy metals in terms of their effect on S. cerevisiae growth, heavy metal (hyper) accumulation, and capacity to remove heavy metal from contaminated environments.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28577027</PMID><DateCompleted><Year>2018</Year><Month>01</Month><Day>18</Day></DateCompleted><DateRevised><Year>2018</Year><Month>01</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN><JournalIssue CitedMedium="Internet"><Volume>101</Volume><Issue>14</Issue><PubDate><Year>2017</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Heavy metal accumulation by Saccharomyces cerevisiae cells armed with metal binding hexapeptides targeted to the inner face of the plasma membrane.</ArticleTitle><Pagination><MedlinePgn>5749-5763</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-017-8335-0</ELocationID><Abstract><AbstractText>Accumulation of heavy metals without developing toxicity symptoms is a phenotype restricted to a small group of plants called hyperaccumulators, whose metal-related characteristics suggested the high potential in biotechnologies such as bioremediation and bioextraction. In an attempt to extrapolate the heavy metal hyperaccumulating phenotype to yeast, we obtained Saccharomyces cerevisiae cells armed with non-natural metal-binding hexapeptides targeted to the inner face of the plasma membrane, expected to sequester the metal ions once they penetrated the cell. We describe the construction of S. cerevisiae strains overexpressing metal-binding hexapeptides (MeBHxP) fused to the carboxy-terminus of a myristoylated green fluorescent protein (myrGFP). Three non-toxic myrGFP-MeBHxP (myrGFP-H6, myrGFP-C6, and myrGFP-(DE)3) were investigated against an array of heavy metals in terms of their effect on S. cerevisiae growth, heavy metal (hyper) accumulation, and capacity to remove heavy metal from contaminated environments.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ruta</LastName><ForeName>Lavinia Liliana</ForeName><Initials>LL</Initials><AffiliationInfo><Affiliation>Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kissen</LastName><ForeName>Ralph</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Cell, Molecular Biology and Genomics Group, Department of Biology, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nicolau</LastName><ForeName>Ioana</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Neagoe</LastName><ForeName>Aurora Daniela</ForeName><Initials>AD</Initials><AffiliationInfo><Affiliation>Faculty of Biology, University of Bucharest, Spl. Independentei 91-95, Bucharest, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Petrescu</LastName><ForeName>Andrei José</ForeName><Initials>AJ</Initials><AffiliationInfo><Affiliation>Institute of Biochemistry of the Romanian Academy, Spl. Independentei 296, Bucharest, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bones</LastName><ForeName>Atle M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Cell, Molecular Biology and Genomics Group, Department of Biology, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Farcasanu</LastName><ForeName>Ileana Cornelia</ForeName><Initials>IC</Initials><AffiliationInfo><Affiliation>Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania. ileana.farcasanu@chimie.unibuc.ro.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>06</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009842">Oligopeptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009842" MajorTopicYN="N">Oligopeptides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Accumulation</Keyword><Keyword MajorTopicYN="N">Heavy metal</Keyword><Keyword MajorTopicYN="N">Metal-binding hexapeptide</Keyword><Keyword MajorTopicYN="N">Saccharomyces cerevisiae</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>02</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>05</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>05</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>6</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>1</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>6</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28577027</ArticleId><ArticleId IdType="doi">10.1007/s00253-017-8335-0</ArticleId><ArticleId IdType="pii">10.1007/s00253-017-8335-0</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Norvège</li><li>Roumanie</li></country><region><li>Trøndelag</li></region><settlement><li>Trondheim</li></settlement></list><tree><country name="Roumanie"><noRegion><name sortKey="Ruta, Lavinia Liliana" sort="Ruta, Lavinia Liliana" uniqKey="Ruta L" first="Lavinia Liliana" last="Ruta">Lavinia Liliana Ruta</name></noRegion><name sortKey="Farcasanu, Ileana Cornelia" sort="Farcasanu, Ileana Cornelia" uniqKey="Farcasanu I" first="Ileana Cornelia" last="Farcasanu">Ileana Cornelia Farcasanu</name><name sortKey="Neagoe, Aurora Daniela" sort="Neagoe, Aurora Daniela" uniqKey="Neagoe A" first="Aurora Daniela" last="Neagoe">Aurora Daniela Neagoe</name><name sortKey="Nicolau, Ioana" sort="Nicolau, Ioana" uniqKey="Nicolau I" first="Ioana" last="Nicolau">Ioana Nicolau</name><name sortKey="Petrescu, Andrei Jose" sort="Petrescu, Andrei Jose" uniqKey="Petrescu A" first="Andrei José" last="Petrescu">Andrei José Petrescu</name></country><country name="Norvège"><region name="Trøndelag"><name sortKey="Kissen, Ralph" sort="Kissen, Ralph" uniqKey="Kissen R" first="Ralph" last="Kissen">Ralph Kissen</name></region><name sortKey="Bones, Atle M" sort="Bones, Atle M" uniqKey="Bones A" first="Atle M" last="Bones">Atle M. 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